Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic and membrane staining of myocytes.Application
Western blot analysis of ALP expression in non-transfected: (A) and human ALP transfected: (B) 293T whole cell lysates and rat skeletal muscle tissue extract (C).WB
b Protein levels of ALP, Runx2, ColI, osteocalcin and TRAP in rat femurs with ovariectomy-induced osteoporosis| Product Name | ALP Antibody (H-157) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | ALP (actinin-associated LIM protein), also known as PDLIM3 (PDZ and LIM domain protein 3) is a 364 amino acid protein that contains one LIM zinc-binding domain and one PDZ domain and localizes to the cytoplasm, as well as to myofiber Z-lines. Existing as three alternatively spliced isoforms, two of which exhibit tissue-specific expression in skeletal muscle and heart, ALP interacts with å-actinin-2 and, via this interaction, is thought to play a role in Actin filament organization, specifically regulating the association of Actin filaments arrays with muscle cells. The gene encoding ALP maps to a region on human chromosome 4 that is associated with facioscapulohumeral muscular dystrophy, suggesting that defects in the ALP gene may be involved in the pathogenesis of muscular dystrophy. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | equine, canine and porcine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 44-200 mapping within an internal region of ALP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 44-200 mapping within an internal region of ALP of human origin |
| Entrez Gene | 27295 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic and membrane staining of myocytes.
Application
Western blot analysis of ALP expression in non-transfected: (A) and human ALP transfected: (B) 293T whole cell lysates and rat skeletal muscle tissue extract (C).
WB
b Protein levels of ALP, Runx2, ColI, osteocalcin and TRAP in rat femurs with ovariectomy-induced osteoporosis| Application Notes | WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
