Application
Western blot analysis of PGDH expression in non-transfected: (A) and mouse PGDH transfected: (B) 293T whole cell lysates.| Product Name | PGDH Antibody (FL-266) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-263 of PGDH of human origin |
| Modification Notes | Prostaglandins are implicated in many physiologic and cellular processes, such as inflammation. NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (PGDH) is the fundamental enzyme of prostaglandin degradation. PGDH, an ubiquitous enzyme, strongly reduces the biologic activity of these molecules by catalyzing the oxidation of the 15-hydroxyl group of prostaglandins to a keto group. Cortisol reduces PGDH activity in human placental cells. 11-b-hydroxysteroid dehydrogenase type II (HSD11B2) converts cortisol to cortisone. In preeclampsia, a disorder characterized by high blood pressure and protein in the urine during pregnancy and the postpartum period, HSD11B2 mRNA expression is reduced, leading to a decrease in HSD11B2 activity. Therefore, the diminished conversion of placental cortisol may lead to reduced PGDH mRNA expression by means of an autocrine or paracrine mechanism. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PGDH of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-263 of PGDH of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-263 of PGDH of human origin |
| Entrez Gene | 3248 |
|---|
Application
Western blot analysis of PGDH expression in non-transfected: (A) and mouse PGDH transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PGDH of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
