Application
Western blot analysis of SUGT1 expression in non-transfected: (A) and mouse SUGT1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SUGT1 expression in non-transfected: (A) and mouse SUGT1 transfected: (B) 293 whole cell lysates.| Product Name | SUGT1 Antibody (C-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of SUGT1 of human origin |
| Modification Notes | SUGT1 (Suppressor of G2 allele of Skp1 p19 homolog, S. cerevisiae), also known as SGT1, is a homolog of the yeast protein Sgt1, a regulator of the cell cycle that is essential for G1/S and G2/M transitions. SUGT1 is a highly soluble protein and shares 26% overall amino acid identity and 30% overall similarity with its yeast counterpart. Localizing to the nucleus and cytoplasm, SUGT1 contains a CS domain, a SGS domain, a p23 domain and three tetratricopeptide repeats (TPR). The function of SUGT1 is conserved across eukaryotes. SUGT1 associates with Skp1 p19 and CUL1, subunits of the SCF (Skp1-Cullin-F-box) ubiquitin ligase complex, and is believed to play a role in protein degradation. In addition, SUGT1 is required for the assembly of kinetochores and functions as a co-chaperone for HSP 90. An additional isoform, SUGT1B (also known as SGT1B), exists for SUGT1 due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFELISA |
| recommended for detection of SUGT1 of mouse, rat and human origin by WB, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of SUGT1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of SUGT1 of human origin |
| Entrez Gene | 10910 |
|---|
Application
Western blot analysis of SUGT1 expression in non-transfected: (A) and mouse SUGT1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SUGT1 expression in non-transfected: (A) and mouse SUGT1 transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of SUGT1 of mouse, rat and human origin by WB, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
