Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing cytoplasmic and nuclear staining of subset of hematopoietic cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in red pulp.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of IL-18 expression in HL-60 (A), human PBL (B), Jurkat (C) and CCRF-CEM (D) whole cell lysates.Application
Western blot analysis of IL-18 expression in mouse liver tissue extract.IF
a Representative image of immunofluorescence for IL-1β and IL-18, scale bars = 20 μm.IF
a Representative image of immunofluorescence for IL-1β and IL-18, scale bars = 20 μm.IHC
b Immunohistochemistry of IL-1β and IL-18; scale bars = 20 μm.IHC
b Immunohistochemistry of IL-1β and IL-18, scale bars = 20 μm.| Product Name | IL-18 Antibody (H-173) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Modification Notes | Four structurally related IL-1 receptor ligands have been described. These include three agonists designated IL-1å, IL-1ß and IL-1g/IL-18 and a specific receptor antagonist, IL-1Rå. IL-1å and IL-1ß play critical roles in the regulation of the immune response and inflammation, serving as activators of T and B lymphocytes and NK (natural killer) cells. IL-18 (also referred to as IL-1g) has been shown to augment the secretion of IFN-g from T lymphocytes and increase NK cell activity in spleen cells. IL-18 exhibits 19% and 12% identity with IL-1å and IL-1ß respectively over the twelve ß-strands of the ß-trefoil fold domain, which is a signature feature of the IL-1 family. The unusual leader sequence of IL-18 may be analogous to the IL-1ß pro-domain which must be cleaved by the serine protease ICE for optimal secretion and biological activity. Originally described as IGIF (IFN-g-inducing factor), IL-18 is induced by mouse liver subsequent to challenge with lipopolysaccharide (LPS). |
| Isotype | IgG |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| See 7 citations for IL-18Antibody (H-173) | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of IL-18 of mouse rat and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of IL-18 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 3606 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing cytoplasmic and nuclear staining of subset of hematopoietic cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in red pulp.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of IL-18 expression in HL-60 (A), human PBL (B), Jurkat (C) and CCRF-CEM (D) whole cell lysates.
Application
Western blot analysis of IL-18 expression in mouse liver tissue extract.
IF
a Representative image of immunofluorescence for IL-1β and IL-18, scale bars = 20 μm.
IF
a Representative image of immunofluorescence for IL-1β and IL-18, scale bars = 20 μm.
IHC
b Immunohistochemistry of IL-1β and IL-18; scale bars = 20 μm.
IHC
b Immunohistochemistry of IL-1β and IL-18, scale bars = 20 μm.| Application Notes | See 7 citations for IL-18Antibody (H-173) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
