Application
Western blot analysis of DIO1 expression in 293T whole cell lysate.| Product Name | DIO1 Antibody (H-76) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | DIO1 (deiodinase, iodothyronine, type I), also known as TXDI1, ITDI1 or 5DI, is a 249 amino acid single-pass membrane protein that localizes to the endoplasmic reticulum and belongs to the iodothyronine deiodinase family. Expressed as nine alternatively spliced isoforms, DIO1 functions as a thiol-dependent propylthiouracil-sensitive oxidoreductase that converts the prohormone thyroxine (T4) to bioactive 3,3',5-triiodothyronine (T3), thereby playing a role in thyroid hormone (TH) activation. Human DIO1 shares 88% sequence similarity with its rat counterpart, suggesting a conserved role between species. The gene encoding DIO1 maps to human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DIO1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 174-249 mapping at the C-terminus of DIO1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 174-249 mapping at the C-terminus of DIO1 of human origin |
| Entrez Gene | 1733 |
|---|
Application
Western blot analysis of DIO1 expression in 293T whole cell lysate.| Application Notes | recommended for detection of DIO1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
