Application
Western blot analysis of IFT122 expression in 721 B whole cell lysate.| Product Name | IFT122 Antibody (E-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of IFT122 of human origin by WB, IP and ELISA |
| Modification Notes | IFT122 (intraflagellar transport 122 homolog) is also known as SPG, WDR10p or WDR10 (WD repeat-containing protein 10) and is a 1,241 amino acid protein that is predominantly expressed in testis and pituitary. IFT122 expression occurs in the germ cells of testis and in the somatic granulosa of ovary, suggesting that IFT122 may be involved in the differentiation of cells within the gonads. IFT122 is localized to the cytoplasm and is expressed as two isoforms. IFT122 contains 7 WD repeats at its N-terminus and is a member of the WD repeat protein family. The WD repeat family consists of functionally diverse regulatory proteins in eukaryotes, involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis and gene regulation. Proteins that contain WD repeats are thought to more easily facilitate the formation of heterotrimeric or multiprotein complexes. In addition to its WD repeats, IFT122 contains an AF-2 domain that functions in transcriptional activation and in the recruitment of coregulatory molecules. Mutations in AF-2 domains are thought to abolish the transcriptional activities of proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of IFT122 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of IFT122 of human origin by WB, IP and ELISA |
| Entrez Gene | 55764 |
|---|
Application
Western blot analysis of IFT122 expression in 721 B whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
