Application
Western blot analysis of OPLAH expression in non-transfected 293T: (A), human OPLAH transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of OPLAH expression in non-transfected 293T: (A), human OPLAH transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of OPLAH expression in K-562 whole cell lysate.| Product Name | OPLAH Antibody (C-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The gamma-glutamyl cycle involves a series of reactions that are necessary for the synthesis and metabolism of glutathione (GST), which is crucial for regulating amino acid transport. OPLAH [5-oxoprolinase (ATP-hydrolysing)], also known as OPLA, 5-oxo-L-prolinase, 5-Opase or DKFZp434H244OPLA, is an enzyme that plays an important role in the gamma-glutamyl cycle by catalyzing the cleavage of 5-oxo-L-proline to form L-glutamate in a reaction coupled to the hydrolysis of ATP to ADP and inorganic phosphate. OPLAH is a 1,288 amino acid protein that exists as a homodimer and belongs to the oxoprolinase family. Expressed at highest levels in kidney, OPLAH has also been found at lower levels in lung, breast, colon and ovary. The gene encoding OPLAH maps to human chromosome 8, which consists of nearly 146 million base pairs, encodes over 800 genes and is associated with a variety of diseases and malignancies including Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome and congenital hypothyroidism. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of OPLAH of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of OPLAH of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of OPLAH of human origin |
| Entrez Gene | 26873 |
|---|
Application
Western blot analysis of OPLAH expression in non-transfected 293T: (A), human OPLAH transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of OPLAH expression in non-transfected 293T: (A), human OPLAH transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of OPLAH expression in K-562 whole cell lysate.| Application Notes | recommended for detection of OPLAH of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
