Application
Western blot analysis of C-Nap1 expression in non-transfected: (A) and human C-Nap1 transfected: (B) 293T whole cell lysates.| Product Name | C-Nap1 Antibody (E-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | C-Nap1 (centrosome-associated protein), also known as Centrosomal Nek2-associated protein 1 or CEP2 (centrosomal protein 2), is localized to the cytoplasm and exists in every human cell. C-Nap1 is expressed as three isoforms and is a 2,442 amino acid protein. C-Nap1 is a centrosomal protein found at the tips of parental centrioles and in basal bodies of cellular projections, such as cillia. C-Nap1 is associated with the interphase stage of the cell cycle, during which C-Nap1 functions in the cohesion of centrioles. The phosphorylation of C-Nap1 plays a role in regulating whether the centrosomes are associating or dissociating. Nek2 phosphorylates the C-terminal end of C-Nap1 during mitosis, an event which is thought to play a role in the dissociation of centrosomes. C-Nap1 reacumulates in centrosomes at the end of cellular division. Some autoimmune diseases are characterized by the production of antibodies against C-Nap1, suggesting that C-Nap1 may be involved in the pathogenesis of autoimmune diseases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of C-Nap1 of mouse and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of C-Nap1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of C-Nap1 of human origin |
| Entrez Gene | 11190 |
|---|
Application
Western blot analysis of C-Nap1 expression in non-transfected: (A) and human C-Nap1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of C-Nap1 of mouse and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
