Application
Western blot analysis of ASAH3 expression in A-431 (A), HeLa (B), K-562 (C), Jurkat (D), SK-MEL-28 (E) and A-375 (F) whole cell lysates.| Product Name | ASAH3 Antibody (N-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | ASAH3 (N-acylsphingosine amidohydrolase (alkaline ceramidase) 3), also designated ACER1, is a 264 amino acid multi-pass membrane protein that localizes to the membrane of the endoplasmic reticulum. Expressed predominately in epidermal tissue, ASAH3 catalyzes the hydrolysis of the sphingolipid ceramide into sphingosine and a free fatty acid, thereby playing an important role in the regulation of bioactive ceramide and sphingosine levels within the cell. ASAH3 functions at an optimal pH of 8.0 and has high specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine, but not D-ribo-phytosphingosine or D-erythro-dihydrosphingosine as a backbone. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of ASAH3 of human origin by WB, IP and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of ASAH3 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of ASAH3 of human origin |
| Entrez Gene | 125981 |
|---|
Application
Western blot analysis of ASAH3 expression in A-431 (A), HeLa (B), K-562 (C), Jurkat (D), SK-MEL-28 (E) and A-375 (F) whole cell lysates.| Application Notes | recommended for detection of ASAH3 of human origin by WB, IP and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
