Application
Western blot analysis of ZNF282 expression in transfected 293T whole cell lysate.| Product Name | ZNF282 Antibody (J-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZNF282 (zinc finger protein 282), also designated HUB1, is a 671 amino acid nuclear protein that contains one KRAB domain and five C2H2-type zinc fingers. Expressed ubiquitously, ZNF282 binds to the 5'-TCCACCCC-3' sequence within the U5 repressive element (U5RE) of the human T cell leukemia virus type I (HTLV-1) long terminal repeat. Through its interaction with the U5RE, ZNF282 effectively represses HTLV-1-mediated expression, thereby suppressing viral replication. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of ZNF282 of mouse, rat and human origin by WB, IP and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against ZNF282 of human origin |
|---|---|
| Tissue Specificity | raised against ZNF282 of human origin |
| Entrez Gene | 8427 |
|---|
Application
Western blot analysis of ZNF282 expression in transfected 293T whole cell lysate.| Application Notes | recommended for detection of ZNF282 of mouse, rat and human origin by WB, IP and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
