Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil carcinoma tissue showing cytoplasmic staining.Application
Western blot analysis of phosphorylated VASP expression in untreated (A) and forskolin-treated (B) NIH/3T3 whole cell lysates.| Product Name | p-VASP Antibody (Ser 157) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Wiskott-Aldrich syndrome (WAS) is characterized by thrombocytopenia, eczema, defects in cell-mediated and humoral immunity and a propensity for lymphoproliferative diseases. The syndrome is the result of a mutation in the gene encoding a proline-rich protein termed WASP. A distantly related protein, VASP (vasodilator-stimulated phosphoprotein), is involved in the maintenance of cytoarchitecture by interacting with actin-like filaments. VASP shares a limited degree of homology with the amino terminus of WASP, which is frequently mutated in WAS patients. An established substrate of cAMP and cGMP dependent kinases, VASP is phosphorylated on a regulatory serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is also phosphorylated on serine 239 by cGMP-dependent protein kinase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 157 phosphorylated VASP of human origin, correspondingly phosphorylated Ser 153 of mouse origin and correspondingly phosphorylated Ser 154 of rat origin by WB, IP, IF and IHC(P) | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 157 of VASP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 157 of VASP of human origin |
| Entrez Gene | 7408 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil carcinoma tissue showing cytoplasmic staining.
Application
Western blot analysis of phosphorylated VASP expression in untreated (A) and forskolin-treated (B) NIH/3T3 whole cell lysates.| Application Notes | recommended for detection of Ser 157 phosphorylated VASP of human origin, correspondingly phosphorylated Ser 153 of mouse origin and correspondingly phosphorylated Ser 154 of rat origin by WB, IP, IF and IHC(P) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
