Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.Application
Western blot analysis of phosphorylated Cdc2 p34 expression in A2780 (A) and MDA-MB-435 (B) whole cell lysates.| Product Name | p-Cdk2 Antibody (Thr 160) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclin dependent kinase 2 (Cdk2) p33, also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Phosphorylation at Thr14 or Tyr15 inactivatesthe enzyme, while phosphorylation at Thr 160 activates it. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Thr 160 phosphorylated Cdk2 of mouse, rat and human origin by WB, IP, IF and IHC(P) | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 160 of Cdk2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 160 of Cdk2 of human origin |
| Entrez Gene | 1017 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.
Application
Western blot analysis of phosphorylated Cdc2 p34 expression in A2780 (A) and MDA-MB-435 (B) whole cell lysates.| Application Notes | recommended for detection of Thr 160 phosphorylated Cdk2 of mouse, rat and human origin by WB, IP, IF and IHC(P) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
