Application
Western blot analysis of DDX54 expression in transfected 293T whole cell lysate.| Product Name | DDX54 Antibody (G-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX54 (DEAD polypeptide 54), also known as DP97, is an 881 amino acid protein that contains two bipartite nuclear localization signals, three nuclear receptor boxes (LXXLL motifs), a potential CoRNR box, and several stretches of glutamate and lysine residues. DDX54 is ubiquitously expressed, with highest expression in pancreas and lung. DDX54 colocalizes with ERå to structures in the nucleoplasm. DDX54 represses ERå transcriptional activity and acts as a nuclear receptor corepressor against ER∫, progesterone, glucocorticoid and RARå. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of DDX54 of human and canine origin by WB, IP and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against DDX54 of human origin |
|---|---|
| Tissue Specificity | raised against DDX54 of human origin |
| Entrez Gene | 79039 |
|---|
Application
Western blot analysis of DDX54 expression in transfected 293T whole cell lysate.| Application Notes | recommended for detection of DDX54 of human and canine origin by WB, IP and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
