WB
(B, C, D) The protein levels of p-53BP1, p-ATM, ATM, γH2AX, and β-actin were detected by Western blotting. Right and down: quantitative analysis of the optical density ratio of p-53BP1, p-ATM, γH2AX compared with the loading control (β-actin) in HCT116 and HCT8.| Product Name | p-53BP1 Antibody (Ser 1778) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Modification Notes | 53BP1, also known as TP53BP1 (tumor protein p53 binding protein 1) or p202, is a 1,972 amino acid protein that is associated with kinetochores and contains two BRCT domains. Existing as multiple alternatively spliced isoforms, 53BP1 interacts with p53 and is thought to play a role in checkpoint signaling during mitosis, possibly enhancing p53-mediated transcriptional activation and influencing the cellular response to DNA damage. 53BP1 is subject to post-translational phosphorylation on specific amino acid residues, including Ser 1778, Ser 25 and Ser 29. The gene encoding 53BP1 maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| WB:1:100~1:500 IF:1:5~1:20 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 1778 of 53BP1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 1778 of 53BP1 of human origin |
| Entrez Gene | 7158 |
|---|
WB
(B, C, D) The protein levels of p-53BP1, p-ATM, ATM, γH2AX, and β-actin were detected by Western blotting. Right and down: quantitative analysis of the optical density ratio of p-53BP1, p-ATM, γH2AX compared with the loading control (β-actin) in HCT116 and HCT8.| Application Notes | WB:1:100~1:500 IF:1:5~1:20 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
