Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach showing cytoplasmic staining of glandular cells at low magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach showing cytoplasmic staining of glandular cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of pyridoxal kinase expression in non-transfected: (A) and mouse pyridoxal kinase transfected: (B) 293T whole cell lysates.Application
Western blot analysis of pyridoxal kinase expression in Hs68 (A) and HeLa (B) whole cell lysates and mouse testis tissue extract (C).| Product Name | pyridoxal kinase Antibody (H-91) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Pyridoxal kinase belongs to the pyridoxine kinase family and phosphorylates vitamin B6, a step necessary for the conversion of vitamin B6 to pyridoxal 5’ phosphate (PLP), the active form of vitamin B6. PLP acts as a coenzyme and functions to maintain homeostasis. Pyridoxal kinase is a 312-amino acid cytoplasmic protein that may act as a homodimer and is expressed ubiquitously. There are three known isoforms of pyridoxal kinase, and isoform 3 expression is observed in adult testis and spermatozoa. The optimum pH for pyridoxal kinase is between 5.5 and 6.0. PDXK, the gene that encodes the pyridoxal kinase protein, maps to chromosome 21q22.3 and may be a candidate gene for autoimmune polyglandular disease type 1, a genetic disorder that has been mapped to the same region on chromosome 21. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of pyridoxal kinase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 221-312 mapping at the C-terminus of pyridoxal kinase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 221-312 mapping at the C-terminus of pyridoxal kinase of human origin |
| Entrez Gene | 8566 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach showing cytoplasmic staining of glandular cells at low magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach showing cytoplasmic staining of glandular cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of pyridoxal kinase expression in non-transfected: (A) and mouse pyridoxal kinase transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of pyridoxal kinase expression in Hs68 (A) and HeLa (B) whole cell lysates and mouse testis tissue extract (C).| Application Notes | recommended for detection of pyridoxal kinase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
