Application
Western blot analysis of MAGE-D4B expression in non-transfected: (A) and human MAGE-D4B transfected: (B) 293T whole cell lysates.| Product Name | MAGE-D4/MAGE-D4B Antibody (H-188) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | MAGE-D4 (Melanoma-associated antigen D4, MAGE-E1 antigen) and MAGE-D4B (Melanoma-associated antigen D4B) are 741 amino acid proteins encoded by the human gene MAGED4 and MAGED4B, respectively. Genes of the MAGE family direct the expression of tumor antigens that are recognized on human melanomas by autologous cytolytic T lymphocytes. MAGE-D4/MAGE-D4B are believed to be glioma-specific members of MAGE family. Among cancer cells, only in glioma cells are both isoforms of MAGE-D4/MAGE-D4B specifically expressed. Among normal tissues, MAGE-D4/MAGE-D4B are expressed only in brain and ovary. Although MAGE-D4/MAGE-D4B are expressed at high levels in malignant tumors as compared to normal tissue, MAGE-D4/MAGE-D4B protein expression is not considered to be of prognostic significance. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MAGE-D4 and MAGE-D4B of human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 23-310 mapping near the N-terminus of MAGE-D4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 23-310 mapping near the N-terminus of MAGE-D4 of human origin |
| Entrez Gene | 728239 |
|---|
Application
Western blot analysis of MAGE-D4B expression in non-transfected: (A) and human MAGE-D4B transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of MAGE-D4 and MAGE-D4B of human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
