| Product Name | HOTTL Antibody (H-124) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | HOTTL (Tubulin-tyrosine ligase-like protein 3) is a 352 amino acid protein encoded by the human gene TTLL3. HOTTL is believed to be part of the Tubulin-tyrosine ligase family and contains one TTL domain. The TTL domain is an approximately 350 amino acid module present in a family of eukaryotic proteins that could catalyze ligations of diverse amino acids to tubulins or other substrates. The TTL domain contains ATP-grasp-like motifs that correspond to the ATP/Mg(2+) binding site typical of enzymes with ATP-dependent carboxylate-amine/thiol ligase activity. Subject to several post-translational modifications, assembled microtubules are detyrosinated over time at the C-terminus of a-tubulin. After microtubular disassembly TTL proteins restore tyrosine residues back to the detyrosinated tubulin leading to a cycle of detyrosination/tyrosination. HOTTL also may play a role in tumor cell regulation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HOTTL of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-124 mapping at the N-terminus of HOTTL of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-124 mapping at the N-terminus of HOTTL of human origin |
| Entrez Gene | 26140 |
|---|
| Application Notes | recommended for detection of HOTTL of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
