Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing membrane and cytoplasmic staining of glandular cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing membrane and cytoplasmic staining of glandular cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of N-SMase2 expression in non-transfected: (A) and mouse N-SMase2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of N-SMase2 expression in PC-12 whole cell lysate.| Product Name | N-SMase2 Antibody (H-195) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | N-SMase2 (neutral sphingomyelinase 2), also known as NSMASE2 or SMPD3 (sphingomyelin phosphodiesterase 3), is a ubiquitously expressed 655 amino acid member of the magnesium-dependent phosphohydrolase protein family. Localized to the membrane of the Golgi apparatus, N-SMase2 functions to catalyze the hydrolysis of sphingomyelin to form ceramide and phosphocholine—two proteins that mediate cell growth arrest and apoptosis. N-SMase2 is enzymatically activated by unsaturated fatty acids and phosphatidylserine and, through regulation of ceramide synthesis, is involved in growth suppression and postnatal development. Expression of N-SMase2 is upregulated during the G0/G1 phases of the cell cycle and optimal N-SMase2 activity occurs at a slightly basic pH of 7.5. N-SMase2 deficiency is the cause of chondrodysplasia, a genetic disorder characterized by impaired bone growth that leads to short stature, bowlegs and underdeveloped joints. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Sphingomyelin phosphodiesterase 3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 461-655 mapping at the C-terminus of N-SMase2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 461-655 mapping at the C-terminus of N-SMase2 of human origin |
| Entrez Gene | 55512 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing membrane and cytoplasmic staining of glandular cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing membrane and cytoplasmic staining of glandular cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of N-SMase2 expression in non-transfected: (A) and mouse N-SMase2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of N-SMase2 expression in PC-12 whole cell lysate.| Application Notes | recommended for detection of Sphingomyelin phosphodiesterase 3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
