Application
MAML1 Antibody (Center) western blot analysis in 293,NCI-H460 cell line lysates (35ug/lane).This demonstrates the MAML1 antibody detected the MAML1 protein (arrow).Application
MAML1 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MAML1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0200 |
|---|---|
| Alternative Names | MAML1 KIAA0200 Mastermind-like protein 1 |
| Molecular Weight(MW) | 108054 Da |
| Function | Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions |
| Tissue Specificity | Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen |
| Cellular Localization | Nucleus speckle. Note=Nuclear, in a punctate manner |
| Entrez Gene | 9794 |
|---|---|
| Nucleotide Accession | NP_055572.1 |
Application
MAML1 Antibody (Center) western blot analysis in 293,NCI-H460 cell line lysates (35ug/lane).This demonstrates the MAML1 antibody detected the MAML1 protein (arrow).
Application
MAML1 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
