Application
Western blot analysis of ERK8 (arrow) using rabbit polyclonal ERK8 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK15 gene (Lane 2) (Origene Technologies).Application
Western blot analysis of anti-ERK8 Antibody (N-term) in T47D cell line lysates (35ug/lane). ERK8 (arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK8 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | MAPK15 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAPK15; ERK7; ERK8; Mitogen-activated protein kinase 15; Extracellular signal-regulated kinase 7; Extracellular signal-regulated kinase 8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ERK7 ERK8 |
|---|---|
| Alternative Names | MAPK15 ERK7 ERK8 Mitogen-activated protein kinase 15 Extracellular signal-regulated kinase 7 Extracellular signal-regulated kinase 8 |
| Molecular Weight(MW) | 59832 Da |
| Function | In vitro, phosphorylates MBP. |
| Tissue Specificity | Widely expressed with a maximal expression in lung and kidney |
| Entrez Gene | 225689 |
|---|
Application
Western blot analysis of ERK8 (arrow) using rabbit polyclonal ERK8 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK15 gene (Lane 2) (Origene Technologies).
Application
Western blot analysis of anti-ERK8 Antibody (N-term) in T47D cell line lysates (35ug/lane). ERK8 (arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK8 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
