Application
Western blot analysis of MARCH5 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane).MARCH5 (arrow) was detected using the purified Pab.Application
MARCH5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MARCH5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
MARCH5 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MARCH5 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MARCH5; RNF153; E3 ubiquitin-protein ligase MARCH5; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; Mitochondrial ubiquitin ligase; RING finger protein 153 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RNF153 |
|---|---|
| Alternative Names | MARCH5 RNF153 E3 ubiquitin-protein ligase MARCH5 Membrane-associated RING finger protein 5 Membrane-associated RING-CH protein V Mitochondrial ubiquitin ligase RING finger protein 153 |
| Molecular Weight(MW) | 31232 Da |
| Function | Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1 |
| Tissue Specificity | Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle |
| Cellular Localization | Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane. Note=Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria (PubMed:16874301) |
Application
Western blot analysis of MARCH5 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane).MARCH5 (arrow) was detected using the purified Pab.
Application
MARCH5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MARCH5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
MARCH5 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
