Application
MARCH1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MARCH1 antibody detected the MARCH1 protein (arrow).| Product Name | MARCH1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MARCH1; RNF171; E3 ubiquitin-protein ligase MARCH1; Membrane-associated RING finger protein 1; Membrane-associated RING-CH protein I; RING finger protein 171 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RNF171 |
|---|---|
| Alternative Names | MARCH1 RNF171 E3 ubiquitin-protein ligase MARCH1 Membrane-associated RING finger protein 1 Membrane-associated RING-CH protein I RING finger protein 171 |
| Molecular Weight(MW) | 32308 Da |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system |
| Tissue Specificity | Expressed in antigen presenting cells, APCs, located in lymph nodes and spleen. Also expressed in lung Expression is high in follicular B-cells, moderate in dendritic cells and low in splenic T-cells |
| Cellular Localization | Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein (By similarity). Lysosome membrane; Multi-pass membrane protein (By similarity). Cytoplasmic vesicle membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein |
| Entrez Gene | 55016 |
|---|---|
| Nucleotide Accession | NP_060393.1 NP_001159845.1 |
Application
MARCH1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MARCH1 antibody detected the MARCH1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
