Application
The anti-MARK1 N-term Pab is used in Western blot to detect MARK1 in P7 mouse whole brain lysate (60 ug). 1:250 dilution of anti-MARK1 pab was used. Data and protocol kindly provided by DR. Shengli Zhao, M.D., Ph.D. of Duke University Medical Center.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with MARK1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | MARK1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MARK1; KIAA1477; MARK; Serine/threonine-protein kinase MARK1; MAP/microtubule affinity-regulating kinase 1; PAR1 homolog c |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1477 MARK |
|---|---|
| Alternative Names | MARK1 KIAA1477 MARK Serine/threonine-protein kinase MARK1 MAP/microtubule affinity-regulating kinase 1 PAR1 homolog c |
| Molecular Weight(MW) | 89003 Da |
| Function | Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2, MAP4 and MAPT/TAU. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3) |
| Tissue Specificity | Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney |
| Cellular Localization | Cell membrane; Peripheral membrane protein. Cytoplasm, cytoskeleton (By similarity). Note=Appears to localize to an intracellular network (By similarity) |
Application
The anti-MARK1 N-term Pab is used in Western blot to detect MARK1 in P7 mouse whole brain lysate (60 ug). 1:250 dilution of anti-MARK1 pab was used. Data and protocol kindly provided by DR. Shengli Zhao, M.D., Ph.D. of Duke University Medical Center.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with MARK1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:200~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
