Application
A. Western blot analysis on Hela cell lysates using anti-MARCKS Phospho (pS159/163) RabMAb (Cat. #AJ1459b), 1:2000 dilution. Cells were either (A) untreated (B) treated with TPA.| Product Name | MARCKS Antibody Phospho (pS159/S163) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MARCKS, MACS, PRKCSL, Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate, 80 kDa protein, light chain |
| Modification | p-S159/S163 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2113Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MACS PRKCSL |
|---|---|
| Alternative Names | MARCKS MACS PRKCSL Myristoylated alanine-rich C-kinase substrate Protein kinase C substrate 80 kDa protein light chain |
| Molecular Weight(MW) | 31555 Da |
| Function | MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding serine 159 and 163 of human MARCKS protein. The orthologous phosphorylation sites in mouse and rat is serine 152 and 156 |
| Cellular Localization | Cytoplasm, cytoskeleton (Probable). Membrane; Lipid-anchor (By similarity) |
| Entrez Gene | 4082 |
|---|
Application
A. Western blot analysis on Hela cell lysates using anti-MARCKS Phospho (pS159/163) RabMAb (Cat. #AJ1459b), 1:2000 dilution. Cells were either (A) untreated (B) treated with TPA.| Application Notes | WB:1:1000~2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
