Application
A. Western blot analysis on melanoma lysate using anti-MART-1 RabMAb (Cat. #AJ1461a), dilution 1:1000.Application
B. Immunohistochemical staining of paraffin-embedded melanoma using anti-MART-1 RabMAb (Cat. #AJ1461a).| Product Name | MART-1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MLANA, MART1, Melanoma antigen recognized by T-cells 1, Antigen SK29-AA;Antigen LB39-AA |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1422Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MART1 |
|---|---|
| Alternative Names | MLANA MART1 Melanoma antigen recognized by T-cells 1 Antigen SK29-AA Antigen LB39-AA |
| Molecular Weight(MW) | 13157 Da |
| Function | Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes |
| Tissue Specificity | Expression is restricted to melanoma and melanocyte cell lines and retina |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Note=Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation |
| Entrez Gene | 2315 |
|---|
Application
A. Western blot analysis on melanoma lysate using anti-MART-1 RabMAb (Cat. #AJ1461a), dilution 1:1000.
Application
B. Immunohistochemical staining of paraffin-embedded melanoma using anti-MART-1 RabMAb (Cat. #AJ1461a).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
