Application
MART-1/Melan-A Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MART-1/Melan-A antibody detected the MART-1/Melan-A protein (arrow).Application
MART-1/Melan-A Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MART-1/Melan-A Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MLANA; MART1; Melanoma antigen recognized by T-cells 1; Antigen LB39-AA; Antigen SK29-AA; Protein Melan-A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MART1 |
|---|---|
| Alternative Names | MLANA MART1 Melanoma antigen recognized by T-cells 1 Antigen LB39-AA Antigen SK29-AA Protein Melan-A |
| Molecular Weight(MW) | 13157 Da |
| Function | Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes |
| Tissue Specificity | Expression is restricted to melanoma and melanocyte cell lines and retina |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Note=Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation |
| Entrez Gene | 2315 |
|---|---|
| Nucleotide Accession | NP_005502.1 |
Application
MART-1/Melan-A Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MART-1/Melan-A antibody detected the MART-1/Melan-A protein (arrow).
Application
MART-1/Melan-A Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
