Application
The anti-MBD2 N-term Antibody is used in Western blot to detect MBD2 in A375 cell lysate (lane 1) and mouse brain tissue lysate (lane 2) lysate.| Product Name | MBD2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MBD2; Methyl-CpG-binding domain protein 2; Demethylase; Methyl-CpG-binding protein MBD2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MBD2 Methyl-CpG-binding domain protein 2 Demethylase Methyl-CpG-binding protein MBD2 |
|---|---|
| Molecular Weight(MW) | 43255 Da |
| Function | Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression May enhance the activation of some unmethylated cAMP-responsive promoters |
| Tissue Specificity | Highly expressed in brain, heart, kidney, stomach, testis and placenta |
| Cellular Localization | Nucleus. Note=Nuclear, in discrete foci. Detected at replication foci in late S phase |
| Entrez Gene | 8932 |
|---|
Application
The anti-MBD2 N-term Antibody is used in Western blot to detect MBD2 in A375 cell lysate (lane 1) and mouse brain tissue lysate (lane 2) lysate.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
