Application
Western blot analysis of MBD3 (arrow) using rabbit polyclonal MBD3 Antibody (C-term C215) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MBD3 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | MBD3 Antibody (C-term C215) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MBD3; Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MBD3 Methyl-CpG-binding domain protein 3 Methyl-CpG-binding protein MBD3 |
|---|---|
| Molecular Weight(MW) | 32844 Da |
| Function | Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing |
| Tissue Specificity | This MBD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208~237 amino acids from the C-terminal region of human MBD3. |
| Cellular Localization | Nucleus. Note=Nuclear, in discrete foci. |
| Entrez Gene | 53615 |
|---|
Application
Western blot analysis of MBD3 (arrow) using rabbit polyclonal MBD3 Antibody (C-term C215) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MBD3 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
