Application
MCAF2 Antibody (Center) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the MCAF2 antibody detected the MCAF2 protein (arrow).Application
MCAF2 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MCAF2 antibody detected the MCAF2 protein (arrow).Application
MCAF2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCAF2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
MCAF2 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MCAF2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATF7IP2; MCAF2; Activating transcription factor 7-interacting protein 2; MBD1-containing chromatin-associated factor 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MCAF2 |
|---|---|
| Alternative Names | ATF7IP2 MCAF2 Activating transcription factor 7-interacting protein 2 MBD1-containing chromatin-associated factor 2 |
| Molecular Weight(MW) | 75764 Da |
| Function | Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. The complex formed with MBD1 and SETDB1 represses transcription and probably couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3) activity (Probable) |
| Tissue Specificity | This MCAF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 325-355 amino acids from the Central region of human MCAF2. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 80063 |
|---|---|
| Nucleotide Accession | NP_079273.2 |
Application
MCAF2 Antibody (Center) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the MCAF2 antibody detected the MCAF2 protein (arrow).
Application
MCAF2 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MCAF2 antibody detected the MCAF2 protein (arrow).
Application
MCAF2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCAF2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
MCAF2 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
