Application
MCF2 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MCF2 antibody detected the MCF2 protein (arrow).Application
MCF2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MCF2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
MCF2 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MCF2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MCF2; DBL; Proto-oncogene DBL; Proto-oncogene MCF-2; MCF2-transforming protein; DBL-transforming protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DBL |
|---|---|
| Alternative Names | MCF2 DBL Proto-oncogene DBL Proto-oncogene MCF-2 MCF2-transforming protein DBL-transforming protein |
| Molecular Weight(MW) | 107673 Da |
| Function | Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42 |
| Tissue Specificity | Isoform 1 is expressed only in brain. Isoform 3 is expressed in heart, kidney, spleen, liver and testis. Isoform 4 is expressed in brain, heart, kidney, testis, placenta, stomach and peripheral blood. The protein is detectable in brain, heart, kidney, intestine, muscle, lung and testis |
| Cellular Localization | Cytoplasm. Isoform 3: Membrane. Note=Colocalizes with CDC42 to plasma membrane |
| Entrez Gene | 4168 |
|---|---|
| Nucleotide Accession | NP_001165349.1 NP_005360.3 |
Application
MCF2 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MCF2 antibody detected the MCF2 protein (arrow).
Application
MCF2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MCF2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
MCF2 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
