Application
MCM8 Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the MCM8 antibody detected the MCM8 protein (arrow).| Product Name | MCM8 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MCM8; C20orf154; DNA replication licensing factor MCM8; Minichromosome maintenance 8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C20orf154 |
|---|---|
| Alternative Names | MCM8 C20orf154 DNA replication licensing factor MCM8 Minichromosome maintenance 8 |
| Molecular Weight(MW) | 93697 Da |
| Function | Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8- MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. May also play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC). Binds chromatin throughout the cell cycle |
| Tissue Specificity | Highest levels in placenta, lung and pancreas. Low levels in skeletal muscle and kidney. Expressed in various tumors with highest levels in colon and lung cancers |
| Cellular Localization | Nucleus. Note=Localizes to nuclear foci and colocalizes with RAD51 |
| Entrez Gene | 84515 |
|---|---|
| Nucleotide Accession | NP_115874.3 NP_877954.1 |
Application
MCM8 Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the MCM8 antibody detected the MCM8 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
