Application
MDFI Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the MDFI antibody detected the MDFI protein (arrow).Application
MDFI Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MDFI Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | MDFI Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MDFI; MyoD family inhibitor; Myogenic repressor I-mf |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MDFI MyoD family inhibitor Myogenic repressor I-mf |
|---|---|
| Molecular Weight(MW) | 25029 Da |
| Function | Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways (By similarity) |
| Tissue Specificity | This MDFI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the Central region of human MDFI. |
| Cellular Localization | Nucleus (By similarity). Cytoplasm (By similarity) |
| Entrez Gene | 4188 |
|---|---|
| Nucleotide Accession | NP_005577.1 |
Application
MDFI Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the MDFI antibody detected the MDFI protein (arrow).
Application
MDFI Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MDFI Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
