Application
Western blot analysis of MDH1 Antibody (C-term) in HL-60(lane 1), HepG2(lane 2) cell line lysates (35ug/lane). MDH1 (arrow) was detected using the purified Pab.Application
Western blot analysis of MDH1 (arrow) using rabbit polyclonal MDH1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MDH1 gene (Lane 2) .Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
MDH1 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MDH1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MDH1; MDHA; Malate dehydrogenase, cytoplasmic; Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MDHA |
|---|---|
| Alternative Names | MDH1 MDHA Malate dehydrogenase cytoplasmic Cytosolic malate dehydrogenase Diiodophenylpyruvate reductase |
| Molecular Weight(MW) | 36426 Da |
| Tissue Specificity | This MDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293~322 amino acids from the C-terminal region of human MDH1. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 4190 |
|---|
Application
Western blot analysis of MDH1 Antibody (C-term) in HL-60(lane 1), HepG2(lane 2) cell line lysates (35ug/lane). MDH1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of MDH1 (arrow) using rabbit polyclonal MDH1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MDH1 gene (Lane 2) .
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
MDH1 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
