Application
Western blot analysis of anti-MDM2 Antibody (T218) pre-incubated with and without blocking peptide in Jurkat cell line lysate. MDM2(arrow) was detected using the purified Pab (1:60 dilution).Application
Confocal immunofluorescent analysis of MDM2 Antibody (T218)(Cat#AP1253d) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).| Product Name | MDM2 Antibody (T218) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MDM2; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Oncoprotein Mdm2; p53-binding protein Mdm2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MDM2 E3 ubiquitin-protein ligase Mdm2 Double minute 2 protein Oncoprotein Mdm2 p53-binding protein Mdm2 |
|---|---|
| Molecular Weight(MW) | 55233 Da |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation |
| Tissue Specificity | Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues |
| Cellular Localization | Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Note=Expressed predominantly in the nucleoplasm Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus |
| Entrez Gene | 4193 |
|---|
Application
Western blot analysis of anti-MDM2 Antibody (T218) pre-incubated with and without blocking peptide in Jurkat cell line lysate. MDM2(arrow) was detected using the purified Pab (1:60 dilution).
Application
Confocal immunofluorescent analysis of MDM2 Antibody (T218)(Cat#AP1253d) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
