Application
MeCP2 Antibody (pS80) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the MeCP2 antibody detected the MeCP2 protein (arrow).Application
MeCP2 Antibody (N-term S80) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MeCP2 Antibody (N-term S80) for immunohistochemistry. Clinical relevance has not been evaluated.Application
MeCP2 Antibody (N-term S80) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MeCP2 Antibody (N-term S80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MECP2; Methyl-CpG-binding protein 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MECP2 Methyl-CpG-binding protein 2 |
|---|---|
| Molecular Weight(MW) | 52441 Da |
| Function | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A |
| Tissue Specificity | Present in all adult somatic tissues tested. |
| Cellular Localization | Nucleus. Note=Colocalized with methyl-CpG in the genome |
| Entrez Gene | 4204 |
|---|---|
| Nucleotide Accession | NP_001104262.1 |
Application
MeCP2 Antibody (pS80) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the MeCP2 antibody detected the MeCP2 protein (arrow).
Application
MeCP2 Antibody (N-term S80) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MeCP2 Antibody (N-term S80) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
MeCP2 Antibody (N-term S80) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
