Application
MECP2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MECP2 antibody detected the MECP2 protein (arrow).Application
MECP2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MECP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | MECP2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MECP2; Methyl-CpG-binding protein 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MECP2 Methyl-CpG-binding protein 2 |
|---|---|
| Molecular Weight(MW) | 52441 Da |
| Function | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A |
| Tissue Specificity | Present in all adult somatic tissues tested. |
| Cellular Localization | Nucleus. Note=Colocalized with methyl-CpG in the genome |
| Entrez Gene | 4204 |
|---|---|
| Nucleotide Accession | NP_004983.1 NP_001104262.1 |
Application
MECP2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MECP2 antibody detected the MECP2 protein (arrow).
Application
MECP2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MECP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
