Application
Western blot analysis of MeCP2-S421* in mouse brain, cerebellum tissue and HepG2 cell line lysates (35ug/lane). CP2 (arrow) was detected using the purified Pab.| Product Name | MeCP2 Antibody (S421) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MECP2; Methyl-CpG-binding protein 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:10~50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MECP2 Methyl-CpG-binding protein 2 |
|---|---|
| Molecular Weight(MW) | 52427 Da |
| Function | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A |
| Tissue Specificity | This MeCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S421 of human MECP2. |
| Cellular Localization | Nucleus (By similarity). Note=Colocalized with methyl-CpG in the genome (By similarity) |
Application
Western blot analysis of MeCP2-S421* in mouse brain, cerebellum tissue and HepG2 cell line lysates (35ug/lane). CP2 (arrow) was detected using the purified Pab.| Application Notes | WB:1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
