Application
ME11A Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ME11A antibody detected the ME11A protein (arrow).Application
ME11A Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ME11A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | ME11A Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NTMT1; C9orf32; METTL11A; NRMT; N-terminal Xaa-Pro-Lys N-methyltransferase 1; Alpha N-terminal protein methyltransferase 1A; Methyltransferase-like protein 11A; N-terminal RCC1 methyltransferase; X-Pro-Lys N-terminal protein methyltransferase 1A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C9orf32 METTL11A NRMT |
|---|---|
| Alternative Names | NTMT1 C9orf32 METTL11A NRMT N-terminal Xaa-Pro-Lys N-methyltransferase 1 Alpha N-terminal protein methyltransferase 1A Methyltransferase-like protein 11A N-terminal RCC1 methyltransferase X-Pro-Lys N-terminal protein methyltransferase 1A |
| Molecular Weight(MW) | 25387 Da |
| Function | Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]- Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di- methylation of Pro in the Pro-Pro-Lys motif. Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1 |
| Tissue Specificity | This ME11A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ME11A. |
| Cellular Localization | Nucleus. Note=Predominantly nuclear. |
| Entrez Gene | 28989 |
|---|---|
| Nucleotide Accession | NP_054783.2 |
Application
ME11A Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ME11A antibody detected the ME11A protein (arrow).
Application
ME11A Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ME11A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
