Application
MED25 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MED25 antibody detected the MED25 protein (arrow).Application
MED25 Antibody (N-term) ( western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MED25 antibody detected the MED25 protein (arrow).Application
MED25 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MED25 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | MED25 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MED25; ACID1; ARC92; PTOV2; Mediator of RNA polymerase II transcription subunit 25; Activator interaction domain-containing protein 1; Activator-recruited cofactor 92 kDa component; Mediator complex subunit 25; p78 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ACID1 ARC92 PTOV2 |
|---|---|
| Alternative Names | MED25 ACID1 ARC92 PTOV2 Mediator of RNA polymerase II transcription subunit 25 Activator interaction domain-containing protein 1 Activator-recruited cofactor 92 kDa component Mediator complex subunit 25 p78 |
| Molecular Weight(MW) | 78171 Da |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription |
| Tissue Specificity | Ubiquitously expressed. Highest levels in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen |
| Cellular Localization | Nucleus. |
| Entrez Gene | 81857 |
|---|---|
| Nucleotide Accession | NP_112235.2 |
Application
MED25 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MED25 antibody detected the MED25 protein (arrow).
Application
MED25 Antibody (N-term) ( western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MED25 antibody detected the MED25 protein (arrow).
Application
MED25 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MED25 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
