Application
MED7 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the MED7 antibody detected the MED7 protein (arrow).Application
MED7 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MED7 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MED7; ARC34; CRSP9; Mediator of RNA polymerase II transcription subunit 7; Activator-recruited cofactor 34 kDa component; Cofactor required for Sp1 transcriptional activation subunit 9; Mediator complex subunit 7; RNA polymerase transcriptional regulation |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ARC34 CRSP9 |
|---|---|
| Alternative Names | MED7 ARC34 CRSP9 Mediator of RNA polymerase II transcription subunit 7 Activator-recruited cofactor 34 kDa component Cofactor required for Sp1 transcriptional activation subunit 9 Mediator complex subunit 7 RNA polymerase transcriptional regulation |
| Molecular Weight(MW) | 27245 Da |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors |
| Tissue Specificity | This MED7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-119 amino acids from the Central region of human MED7. |
| Cellular Localization | Nucleus. |
Application
MED7 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the MED7 antibody detected the MED7 protein (arrow).
Application
MED7 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
