WB
Western blot analysis of ARPC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell lysate, Lane 2: Jurkat cell lysate, Lane 3: Mouse lung tissue lysate, Lane 4: Mouse kidney tissue lysate.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ARPC2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ARPC2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | ARPC2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human ARPC2 aa 201-300/300. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ARPC2 |
|---|---|
| Gene Synonyms | ARC34 PRO2446 p34-Arc PNAS-139 |
| Gene Full Name | actin related protein 2/3 complex subunit 2 |
| Gene Summary | This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined. [provided by RefSeq, Jul 2008] |
| Alternative Names | Actin related protein 2/3 complex subunit 2 34kDa antibody Actin related protein 2/3 complex subunit 2 antibody Actin-related protein 2/3 complex subunit 2 antibody ARC 34 antibody ARC34 antibody Arp2/3 complex 34 kDa subunit antibody ARP2/3 protein compex subunit 34 antibody ARPC 2 antibody Arpc2 antibody ARPC2_HUMAN antibody Actin related protein 2/3 complex subunit 2 34kDa antibody Actin related protein 2/3 complex subunit 2 antibody Actin-related protein 2/3 complex subunit 2 antibody ARC 34 antibody ARC34 antibody Arp2/3 complex 34 kDa subunit antibody ARP2/3 protein compex subunit 34 antibody ARPC 2 antibody Arpc2 antibody ARPC2_HUMAN antibody p34 Arc antibody p34-ARC antibody p34Arc antibody PNAS 139 antibody PNAS139 antibody PRO 2446 antibody PRO2446 antibody |
| Molecular Weight(MW) | 34kDa |
| Cellular Localization | Nucleus, Cytoskeleton, Cell projection, Synaptosome. |
WB
Western blot analysis of ARPC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell lysate, Lane 2: Jurkat cell lysate, Lane 3: Mouse lung tissue lysate, Lane 4: Mouse kidney tissue lysate.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ARPC2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ARPC2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
