Application
MED8 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MED8 antibody detected the MED8 protein (arrow).| Product Name | MED8 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MED8; Mediator of RNA polymerase II transcription subunit 8; Activator-recruited cofactor 32 kDa component; Mediator complex subunit 8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MED8 Mediator of RNA polymerase II transcription subunit 8 Activator-recruited cofactor 32 kDa component Mediator complex subunit 8 |
|---|---|
| Molecular Weight(MW) | 29080 Da |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins |
| Tissue Specificity | This MED8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-130 amino acids from the Central region of human MED8. |
| Cellular Localization | Nucleus (Probable). |
| Entrez Gene | 112950 |
|---|---|
| Nucleotide Accession | NP_443109.2 NP_963836.2 |
Application
MED8 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MED8 antibody detected the MED8 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
