Application
MED4 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the MED4 antibody detected the MED4 protein (arrow).Application
MED4 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MED4 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
MED4 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MED4 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MED4; ARC36; DRIP36; VDRIP; Mediator of RNA polymerase II transcription subunit 4; Activator-recruited cofactor 36 kDa component; Mediator complex subunit 4; TRAP/SMCC/PC2 subunit p36 subunit; Vitamin D3 receptor-interacting protein complex 36 kDa componen |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ARC36 DRIP36 VDRIP |
|---|---|
| Alternative Names | MED4 ARC36 DRIP36 VDRIP Mediator of RNA polymerase II transcription subunit 4 Activator-recruited cofactor 36 kDa component Mediator complex subunit 4 TRAP/SMCC/PC2 subunit p36 subunit Vitamin D3 receptor-interacting protein complex 36 kDa componen |
| Molecular Weight(MW) | 29745 Da |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors |
| Tissue Specificity | This MED4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-256 amino acids from the C-terminal region of human MED4. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 29079 |
|---|---|
| Nucleotide Accession | NP_054885.1 |
Application
MED4 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the MED4 antibody detected the MED4 protein (arrow).
Application
MED4 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MED4 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
MED4 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
