Application
MEIS2 Antibody (Center D269) western blot analysis in WiDr cell line lysates (15ug/lane).This demonstrates the MEIS2 antibody detected the MEIS2 protein (arrow).Application
MEIS2 Antibody (Center D269) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MEIS2 Antibody (Center D269) for immunohistochemistry. Clinical relevance has not been evaluated.Application
MEIS2 Antibody (Center D269) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MEIS2 Antibody (Center D269) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MEIS2; MRG1; Homeobox protein Meis2; Meis1-related protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MRG1 |
|---|---|
| Alternative Names | MEIS2 MRG1 Homeobox protein Meis2 Meis1-related protein 1 |
| Molecular Weight(MW) | 51790 Da |
| Function | Involved in transcriptional regulation. Binds to HOX or PBX proteins to form dimers, or to a DNA-bound dimer of PBX and HOX proteins and thought to have a role in stabilization of the homeoprotein-DNA complex. Isoform 3/Meis2B is required for the activity of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element; MEIS2 is not involved in complex DNA-binding. Probably in complex with PBX1, is involved in transcriptional regulation by KLF4. Isoform 3/Meis2B and isoform 4/Meis2D can bind to a EPHA8 promoter sequence containing the DNA motif 5'-CGGTCA-3'; in cooperation with a PBX protein (such as PBX2) is proposed to be involved in the transcriptional activation of EPHA8 in the developing midbrain. May be involved in regulation of myeloid differentiation. Can bind to the DNA sequence 5'-TGACAG-3'in the activator ACT sequence of the D(1A) dopamine receptor (DRD1) promoter and activate DRD1 transcription; isoform 5/Meis2E cannot activate DRD1 transcription |
| Tissue Specificity | Expressed in various tissues. Expressed at high level in the lymphoid organs of hematopoietic tissues. Also expressed in some regions of the brain, such as the putamen |
| Cellular Localization | Nucleus (By similarity). |
Application
MEIS2 Antibody (Center D269) western blot analysis in WiDr cell line lysates (15ug/lane).This demonstrates the MEIS2 antibody detected the MEIS2 protein (arrow).
Application
MEIS2 Antibody (Center D269) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MEIS2 Antibody (Center D269) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
MEIS2 Antibody (Center D269) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
