Application
CITED2 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).This demonstrates the CITED2 antibody detected CITED2 protein (arrow).Application
Confocal immunofluorescent analysis of CITED2 Antibody (C-term) with Hela cell followed by Alexa Fluor® 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | CITED2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CITED2; MRG1; Cbp/p300-interacting transactivator 2; MSG-related protein 1; P35srj |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MRG1 |
|---|---|
| Alternative Names | CITED2 MRG1 Cbp/p300-interacting transactivator 2 MSG-related protein 1 P35srj |
| Molecular Weight(MW) | 28497 Da |
| Function | Transcriptional coactivator of the p300/CBP-mediated trancription complex. Acts as a bridge, linking TFAP2 transcription factors and the p300/CBP transcriptional coactivator complex in order to stimulate TFAP2-mediated transcriptional activation. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Stimulates the peroxisome proliferator- activated receptors PPARA transcriptional activity. Enhances estrogen-dependent transactivation mediated by estrogen receptors Acts also as a transcriptional corepressor; interferes with the binding of the transcription factors HIF1A or STAT2 and the p300/CBP transcriptional coactivator complex. Participates in sex determination and early gonad development by stimulating transcription activation of SRY. Plays a role in controlling left- right patterning during embryogenesis; potentiates transcriptional activation of NODAL-mediated gene transcription in the left lateral plate mesoderm (LPM). Plays an essential role in differentiation of the adrenal cortex from the adrenogonadal primordium (AGP); stimulates WT1-mediated transcription activation thereby up-regulating the nuclear hormone receptor NR5A1 promoter activity. Associates with chromatin to the PITX2 P1 promoter region |
| Tissue Specificity | This CITED2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 207-234 amino acids from the C-terminal region of human CITED2. |
| Cellular Localization | Nucleus. Note=Colocalizes with EP300 in dot- like structures |
| Entrez Gene | 10370 |
|---|---|
| Nucleotide Accession | NP_001161860.1 NP_001161861.1 NP_006070.2 |
Application
CITED2 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).This demonstrates the CITED2 antibody detected CITED2 protein (arrow).
Application
Confocal immunofluorescent analysis of CITED2 Antibody (C-term) with Hela cell followed by Alexa Fluor® 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
