Application
A. Western blot analysis on Hela cell lysate using anti-MEKK-2 (C-term) RabMAb (Cat. #AJ1470b), dilution 1:1,000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-MEKK2 RabMAb (Cat. #AJ1470b).| Product Name | MEKK2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP3K2, MAPKKK2, MEKK2, Mitogen-activated protein kinase kinase kinase 2, MAPK/ERK kinase kinase 2 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP550Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAPKKK2 MEKK2 |
|---|---|
| Alternative Names | MAP3K2 MAPKKK2 MEKK2 Mitogen-activated protein kinase kinase kinase 2 MAPK/ERK kinase kinase 2 |
| Molecular Weight(MW) | 69741 Da |
| Function | Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics |
| Tissue Specificity | A synthetic peptide corresponding to residues in the C-term of human MEKK-2 was used as immunogen. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Upon EGF stimulation, translocates into the nucleus |
| Entrez Gene | 10746 |
|---|
Application
A. Western blot analysis on Hela cell lysate using anti-MEKK-2 (C-term) RabMAb (Cat. #AJ1470b), dilution 1:1,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-MEKK2 RabMAb (Cat. #AJ1470b).| Application Notes | WB:1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
