Application
The anti-MELK Pab is used in Western blot to detect MKEL in HEPG2 tissue lysate| Product Name | MELK Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MELK; KIAA0175; Maternal embryonic leucine zipper kinase; Protein kinase Eg3; Protein kinase PK38; Tyrosine-protein kinase MELK |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0175 |
|---|---|
| Alternative Names | MELK KIAA0175 Maternal embryonic leucine zipper kinase Protein kinase Eg3 Protein kinase PK38 Tyrosine-protein kinase MELK |
| Molecular Weight(MW) | 74642 Da |
| Function | Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis Plays a key role in cell proliferation and carcinogenesis Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis |
| Tissue Specificity | Expressed in placenta, kidney, thymus, testis, ovary and intestine |
| Cellular Localization | Cell membrane; Peripheral membrane protein. |
| Entrez Gene | 9833 |
|---|
Application
The anti-MELK Pab is used in Western blot to detect MKEL in HEPG2 tissue lysate| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
