Application
Dot blot analysis of anti-Methyl-H3-mK9(2Me) Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Methyl-H3-mK9(2Me) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H3F3C; Histone H3.3C; Histone H3.5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | H3F3C Histone H3.3C Histone H3.5 |
|---|---|
| Molecular Weight(MW) | 15214 Da |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Hominid-specific H3.5/H3F3C preferentially colocalizes with euchromatin, and it is associated with actively transcribed genes |
| Tissue Specificity | Specifically expressed in the seminiferous tubules of testis |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 440093 |
|---|
Application
Dot blot analysis of anti-Methyl-H3-mK9(2Me) Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step methylspecific peptide affinity purification. |
