Application
Western blot analysis of MFN2 Antibody (Center) in K562 cell line lysates (35ug/lane). MFN2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded mouse kidney reacted with MFN2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
MFN2 Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MFN2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MFN2; CPRP1; KIAA0214; Mitofusin-2; Transmembrane GTPase MFN2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100-500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CPRP1 KIAA0214 |
|---|---|
| Alternative Names | MFN2 CPRP1 KIAA0214 Mitofusin-2 Transmembrane GTPase MFN2 |
| Molecular Weight(MW) | 86402 Da |
| Function | Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation |
| Tissue Specificity | Ubiquitous; expressed at low level. Highly expressed in heart and kidney |
| Cellular Localization | Mitochondrion outer membrane; Multi-pass membrane protein. Note=Colocalizes with BAX during apoptosis |
| Entrez Gene | 9927 |
|---|
Application
Western blot analysis of MFN2 Antibody (Center) in K562 cell line lysates (35ug/lane). MFN2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded mouse kidney reacted with MFN2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
MFN2 Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100-500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
