Application
Western blot analysis of MFRN2 Antibody (N-term) in Jurkat cell line lysates (35ug/lane).MFRN2 (arrow) was detected using the purified Pab.Application
MFRN2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MFRN2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
MFRN2 Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MFRN2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SLC25A28; MFRN2; Mitoferrin-2; Mitochondrial RNA-splicing protein 3/4 homolog; Mitochondrial iron transporter 2; Solute carrier family 25 member 28 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MFRN2 |
|---|---|
| Alternative Names | SLC25A28 MFRN2 Mitoferrin-2 Mitochondrial RNA-splicing protein 3/4 homolog Mitochondrial iron transporter 2 Solute carrier family 25 member 28 |
| Molecular Weight(MW) | 39272 Da |
| Function | Mitochondrial iron transporter that mediates iron uptake. Probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells. The iron delivered into the mitochondria, presumably as Fe(2+), is then probably delivered to ferrochelatase to catalyze Fe(2+) incorporation into protoprophyrin IX to make heme (By similarity) |
| Tissue Specificity | Ubiquitous. Expressed in placenta, lung, kidney, pancreas, liver, brain, skeletal muscle and heart |
| Cellular Localization | Mitochondrion inner membrane; Multi-pass membrane protein. Note=Isoform 1 and isoform 2 are both localized in the mitochondrion |
| Entrez Gene | 81894 |
|---|
Application
Western blot analysis of MFRN2 Antibody (N-term) in Jurkat cell line lysates (35ug/lane).MFRN2 (arrow) was detected using the purified Pab.
Application
MFRN2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MFRN2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
MFRN2 Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
